human anti cbx3 Search Results


anti cbx3  (Proteintech)
93
Proteintech anti cbx3
Anti Cbx3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cbx3/product/Proteintech
Average 93 stars, based on 1 article reviews
anti cbx3 - by Bioz Stars, 2026-03
93/100 stars
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anti-cbx3 antibody  (Wuhan Sanying Biotechnology)
90
Wuhan Sanying Biotechnology anti-cbx3 antibody
Anti Cbx3 Antibody, supplied by Wuhan Sanying Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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hp1γ cbx3 immunolabeling  (Bethyl)
91
Bethyl hp1γ cbx3 immunolabeling
Persistent hyperemic foci exhibit evidence of dermal senescence. ( A – E ) Dermal cells in areas of high Hgb content exhibit a heterochromatin pattern of DAPI staining. After excision of skin from mice treated with and without UV at 2 and 20 weeks after stopping UV treatments, the sections were stained with DAPI to better demonstrate nuclear morphology. Nuclei of dermal cells showing a heterochromatin staining pattern are shown by white arrows while nuclei exhibiting a euchromatin pattern of DAPI staining are shown by yellow arrows. The hatched white line outlines the epidermal-dermal junction. The white scale bar represents 50 µm. ( A ) Skin from an area of low Hgb content 2 weeks after stopping UV treatments. ( B ) Skin from an area of high Hgb content 2 weeks after stopping UV treatments. ( C ) Control non-UV treated skin. ( D ) Skin from an area of low Hgb content 20 weeks after stopping UV. ( E ) Skin from an area of high Hgb content 20 weeks after stopping UV. (F – J ) Representative photomigrographs of skin following <t>immunolabeling</t> with anti-p16 INK4a antibodies. ( F ) Control non-UV treated epidermis. ( G , H ) Skin excised 2 weeks after stopping UV treatments from an area of low Hgb content ( G ) or from an area of high Hgb content ( H ). ( I , J ) Skin excised from a low Hgb area ( I ) or a high Hgb area ( J ) at 20 weeks after stopping UV treatments. Black arrows in ( H , J ) show dermal cells with enlarged nuclei labeling positive for p16 INK4a . The black scale bars represent 100 µm. ( K ) <t>HP1γ</t> + dermal cells are increased in hyperemic areas at both 2 and 20 weeks after stopping UV treatments. Immunofluorescent (IF) labeling of formalin-fixed skin sections was performed using anti-HP1γ and anti-pancytokeratin (CK) antibody. The data depicts the % of dermal cells positive for HP1γ nuclear labeling. ( L ) Dermal cells positive for nuclear γH2AX are also increased in hyperemic areas only at 2 and 20 weeks post-UV. IF was performed for both nuclear γH2AX immunolabeling and CK. The data shown is the percentage of γH2AX + dermal cells relative to all CK negative cells. (ns = non-significant; ** = p < 0.01; *** = p < 0.001).
Hp1γ Cbx3 Immunolabeling, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
hp1γ cbx3 immunolabeling - by Bioz Stars, 2026-03
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rabbit anti cbx3  (Bethyl)
91
Bethyl rabbit anti cbx3
Persistent hyperemic foci exhibit evidence of dermal senescence. ( A – E ) Dermal cells in areas of high Hgb content exhibit a heterochromatin pattern of DAPI staining. After excision of skin from mice treated with and without UV at 2 and 20 weeks after stopping UV treatments, the sections were stained with DAPI to better demonstrate nuclear morphology. Nuclei of dermal cells showing a heterochromatin staining pattern are shown by white arrows while nuclei exhibiting a euchromatin pattern of DAPI staining are shown by yellow arrows. The hatched white line outlines the epidermal-dermal junction. The white scale bar represents 50 µm. ( A ) Skin from an area of low Hgb content 2 weeks after stopping UV treatments. ( B ) Skin from an area of high Hgb content 2 weeks after stopping UV treatments. ( C ) Control non-UV treated skin. ( D ) Skin from an area of low Hgb content 20 weeks after stopping UV. ( E ) Skin from an area of high Hgb content 20 weeks after stopping UV. (F – J ) Representative photomigrographs of skin following <t>immunolabeling</t> with anti-p16 INK4a antibodies. ( F ) Control non-UV treated epidermis. ( G , H ) Skin excised 2 weeks after stopping UV treatments from an area of low Hgb content ( G ) or from an area of high Hgb content ( H ). ( I , J ) Skin excised from a low Hgb area ( I ) or a high Hgb area ( J ) at 20 weeks after stopping UV treatments. Black arrows in ( H , J ) show dermal cells with enlarged nuclei labeling positive for p16 INK4a . The black scale bars represent 100 µm. ( K ) <t>HP1γ</t> + dermal cells are increased in hyperemic areas at both 2 and 20 weeks after stopping UV treatments. Immunofluorescent (IF) labeling of formalin-fixed skin sections was performed using anti-HP1γ and anti-pancytokeratin (CK) antibody. The data depicts the % of dermal cells positive for HP1γ nuclear labeling. ( L ) Dermal cells positive for nuclear γH2AX are also increased in hyperemic areas only at 2 and 20 weeks post-UV. IF was performed for both nuclear γH2AX immunolabeling and CK. The data shown is the percentage of γH2AX + dermal cells relative to all CK negative cells. (ns = non-significant; ** = p < 0.01; *** = p < 0.001).
Rabbit Anti Cbx3, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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anti cbx3  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti cbx3
Persistent hyperemic foci exhibit evidence of dermal senescence. ( A – E ) Dermal cells in areas of high Hgb content exhibit a heterochromatin pattern of DAPI staining. After excision of skin from mice treated with and without UV at 2 and 20 weeks after stopping UV treatments, the sections were stained with DAPI to better demonstrate nuclear morphology. Nuclei of dermal cells showing a heterochromatin staining pattern are shown by white arrows while nuclei exhibiting a euchromatin pattern of DAPI staining are shown by yellow arrows. The hatched white line outlines the epidermal-dermal junction. The white scale bar represents 50 µm. ( A ) Skin from an area of low Hgb content 2 weeks after stopping UV treatments. ( B ) Skin from an area of high Hgb content 2 weeks after stopping UV treatments. ( C ) Control non-UV treated skin. ( D ) Skin from an area of low Hgb content 20 weeks after stopping UV. ( E ) Skin from an area of high Hgb content 20 weeks after stopping UV. (F – J ) Representative photomigrographs of skin following <t>immunolabeling</t> with anti-p16 INK4a antibodies. ( F ) Control non-UV treated epidermis. ( G , H ) Skin excised 2 weeks after stopping UV treatments from an area of low Hgb content ( G ) or from an area of high Hgb content ( H ). ( I , J ) Skin excised from a low Hgb area ( I ) or a high Hgb area ( J ) at 20 weeks after stopping UV treatments. Black arrows in ( H , J ) show dermal cells with enlarged nuclei labeling positive for p16 INK4a . The black scale bars represent 100 µm. ( K ) <t>HP1γ</t> + dermal cells are increased in hyperemic areas at both 2 and 20 weeks after stopping UV treatments. Immunofluorescent (IF) labeling of formalin-fixed skin sections was performed using anti-HP1γ and anti-pancytokeratin (CK) antibody. The data depicts the % of dermal cells positive for HP1γ nuclear labeling. ( L ) Dermal cells positive for nuclear γH2AX are also increased in hyperemic areas only at 2 and 20 weeks post-UV. IF was performed for both nuclear γH2AX immunolabeling and CK. The data shown is the percentage of γH2AX + dermal cells relative to all CK negative cells. (ns = non-significant; ** = p < 0.01; *** = p < 0.001).
Anti Cbx3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cbx3/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
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mouse anti-hp1 antibodies anti-cbx5 2hp-1h5  (Euromedex)
90
Euromedex mouse anti-hp1 antibodies anti-cbx5 2hp-1h5
Persistent hyperemic foci exhibit evidence of dermal senescence. ( A – E ) Dermal cells in areas of high Hgb content exhibit a heterochromatin pattern of DAPI staining. After excision of skin from mice treated with and without UV at 2 and 20 weeks after stopping UV treatments, the sections were stained with DAPI to better demonstrate nuclear morphology. Nuclei of dermal cells showing a heterochromatin staining pattern are shown by white arrows while nuclei exhibiting a euchromatin pattern of DAPI staining are shown by yellow arrows. The hatched white line outlines the epidermal-dermal junction. The white scale bar represents 50 µm. ( A ) Skin from an area of low Hgb content 2 weeks after stopping UV treatments. ( B ) Skin from an area of high Hgb content 2 weeks after stopping UV treatments. ( C ) Control non-UV treated skin. ( D ) Skin from an area of low Hgb content 20 weeks after stopping UV. ( E ) Skin from an area of high Hgb content 20 weeks after stopping UV. (F – J ) Representative photomigrographs of skin following <t>immunolabeling</t> with anti-p16 INK4a antibodies. ( F ) Control non-UV treated epidermis. ( G , H ) Skin excised 2 weeks after stopping UV treatments from an area of low Hgb content ( G ) or from an area of high Hgb content ( H ). ( I , J ) Skin excised from a low Hgb area ( I ) or a high Hgb area ( J ) at 20 weeks after stopping UV treatments. Black arrows in ( H , J ) show dermal cells with enlarged nuclei labeling positive for p16 INK4a . The black scale bars represent 100 µm. ( K ) <t>HP1γ</t> + dermal cells are increased in hyperemic areas at both 2 and 20 weeks after stopping UV treatments. Immunofluorescent (IF) labeling of formalin-fixed skin sections was performed using anti-HP1γ and anti-pancytokeratin (CK) antibody. The data depicts the % of dermal cells positive for HP1γ nuclear labeling. ( L ) Dermal cells positive for nuclear γH2AX are also increased in hyperemic areas only at 2 and 20 weeks post-UV. IF was performed for both nuclear γH2AX immunolabeling and CK. The data shown is the percentage of γH2AX + dermal cells relative to all CK negative cells. (ns = non-significant; ** = p < 0.01; *** = p < 0.001).
Mouse Anti Hp1 Antibodies Anti Cbx5 2hp 1h5, supplied by Euromedex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-hp1 antibodies anti-cbx5 2hp-1h5/product/Euromedex
Average 90 stars, based on 1 article reviews
mouse anti-hp1 antibodies anti-cbx5 2hp-1h5 - by Bioz Stars, 2026-03
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anti phospho cbx3 ser93  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti phospho cbx3 ser93
Persistent hyperemic foci exhibit evidence of dermal senescence. ( A – E ) Dermal cells in areas of high Hgb content exhibit a heterochromatin pattern of DAPI staining. After excision of skin from mice treated with and without UV at 2 and 20 weeks after stopping UV treatments, the sections were stained with DAPI to better demonstrate nuclear morphology. Nuclei of dermal cells showing a heterochromatin staining pattern are shown by white arrows while nuclei exhibiting a euchromatin pattern of DAPI staining are shown by yellow arrows. The hatched white line outlines the epidermal-dermal junction. The white scale bar represents 50 µm. ( A ) Skin from an area of low Hgb content 2 weeks after stopping UV treatments. ( B ) Skin from an area of high Hgb content 2 weeks after stopping UV treatments. ( C ) Control non-UV treated skin. ( D ) Skin from an area of low Hgb content 20 weeks after stopping UV. ( E ) Skin from an area of high Hgb content 20 weeks after stopping UV. (F – J ) Representative photomigrographs of skin following <t>immunolabeling</t> with anti-p16 INK4a antibodies. ( F ) Control non-UV treated epidermis. ( G , H ) Skin excised 2 weeks after stopping UV treatments from an area of low Hgb content ( G ) or from an area of high Hgb content ( H ). ( I , J ) Skin excised from a low Hgb area ( I ) or a high Hgb area ( J ) at 20 weeks after stopping UV treatments. Black arrows in ( H , J ) show dermal cells with enlarged nuclei labeling positive for p16 INK4a . The black scale bars represent 100 µm. ( K ) <t>HP1γ</t> + dermal cells are increased in hyperemic areas at both 2 and 20 weeks after stopping UV treatments. Immunofluorescent (IF) labeling of formalin-fixed skin sections was performed using anti-HP1γ and anti-pancytokeratin (CK) antibody. The data depicts the % of dermal cells positive for HP1γ nuclear labeling. ( L ) Dermal cells positive for nuclear γH2AX are also increased in hyperemic areas only at 2 and 20 weeks post-UV. IF was performed for both nuclear γH2AX immunolabeling and CK. The data shown is the percentage of γH2AX + dermal cells relative to all CK negative cells. (ns = non-significant; ** = p < 0.01; *** = p < 0.001).
Anti Phospho Cbx3 Ser93, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
anti phospho cbx3 ser93 - by Bioz Stars, 2026-03
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polyclonal rabbit anti-cbx-3 (chromobox protein homolog 3  (Millipore)
90
Millipore polyclonal rabbit anti-cbx-3 (chromobox protein homolog 3
In vitro validation of differentially expressed proteins. In line with the results of the SILAC method and mass spectrometry, CD81 and <t>CBX-3</t> show markedly higher expression in TCam-2 than in NTERA-2 (a, b). Conversely, PHF-6 and ENSA show markedly higher expression in NTERA-2 than in TCam-2 cells (c, d).
Polyclonal Rabbit Anti Cbx 3 (Chromobox Protein Homolog 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
polyclonal rabbit anti-cbx-3 (chromobox protein homolog 3 - by Bioz Stars, 2026-03
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anti mdig  (Proteintech)
91
Proteintech anti mdig
In vitro validation of differentially expressed proteins. In line with the results of the SILAC method and mass spectrometry, CD81 and <t>CBX-3</t> show markedly higher expression in TCam-2 than in NTERA-2 (a, b). Conversely, PHF-6 and ENSA show markedly higher expression in NTERA-2 than in TCam-2 cells (c, d).
Anti Mdig, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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anti flag  (Proteintech)
96
Proteintech anti flag
In vitro validation of differentially expressed proteins. In line with the results of the SILAC method and mass spectrometry, CD81 and <t>CBX-3</t> show markedly higher expression in TCam-2 than in NTERA-2 (a, b). Conversely, PHF-6 and ENSA show markedly higher expression in NTERA-2 than in TCam-2 cells (c, d).
Anti Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
anti flag - by Bioz Stars, 2026-03
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biotinylated anti mouse albumin ab  (Bethyl)
94
Bethyl biotinylated anti mouse albumin ab
In vitro validation of differentially expressed proteins. In line with the results of the SILAC method and mass spectrometry, CD81 and <t>CBX-3</t> show markedly higher expression in TCam-2 than in NTERA-2 (a, b). Conversely, PHF-6 and ENSA show markedly higher expression in NTERA-2 than in TCam-2 cells (c, d).
Biotinylated Anti Mouse Albumin Ab, supplied by Bethyl, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated anti mouse albumin ab - by Bioz Stars, 2026-03
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streptavidin conjugated with hrp  (SouthernBiotech)
96
SouthernBiotech streptavidin conjugated with hrp
In vitro validation of differentially expressed proteins. In line with the results of the SILAC method and mass spectrometry, CD81 and <t>CBX-3</t> show markedly higher expression in TCam-2 than in NTERA-2 (a, b). Conversely, PHF-6 and ENSA show markedly higher expression in NTERA-2 than in TCam-2 cells (c, d).
Streptavidin Conjugated With Hrp, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Persistent hyperemic foci exhibit evidence of dermal senescence. ( A – E ) Dermal cells in areas of high Hgb content exhibit a heterochromatin pattern of DAPI staining. After excision of skin from mice treated with and without UV at 2 and 20 weeks after stopping UV treatments, the sections were stained with DAPI to better demonstrate nuclear morphology. Nuclei of dermal cells showing a heterochromatin staining pattern are shown by white arrows while nuclei exhibiting a euchromatin pattern of DAPI staining are shown by yellow arrows. The hatched white line outlines the epidermal-dermal junction. The white scale bar represents 50 µm. ( A ) Skin from an area of low Hgb content 2 weeks after stopping UV treatments. ( B ) Skin from an area of high Hgb content 2 weeks after stopping UV treatments. ( C ) Control non-UV treated skin. ( D ) Skin from an area of low Hgb content 20 weeks after stopping UV. ( E ) Skin from an area of high Hgb content 20 weeks after stopping UV. (F – J ) Representative photomigrographs of skin following immunolabeling with anti-p16 INK4a antibodies. ( F ) Control non-UV treated epidermis. ( G , H ) Skin excised 2 weeks after stopping UV treatments from an area of low Hgb content ( G ) or from an area of high Hgb content ( H ). ( I , J ) Skin excised from a low Hgb area ( I ) or a high Hgb area ( J ) at 20 weeks after stopping UV treatments. Black arrows in ( H , J ) show dermal cells with enlarged nuclei labeling positive for p16 INK4a . The black scale bars represent 100 µm. ( K ) HP1γ + dermal cells are increased in hyperemic areas at both 2 and 20 weeks after stopping UV treatments. Immunofluorescent (IF) labeling of formalin-fixed skin sections was performed using anti-HP1γ and anti-pancytokeratin (CK) antibody. The data depicts the % of dermal cells positive for HP1γ nuclear labeling. ( L ) Dermal cells positive for nuclear γH2AX are also increased in hyperemic areas only at 2 and 20 weeks post-UV. IF was performed for both nuclear γH2AX immunolabeling and CK. The data shown is the percentage of γH2AX + dermal cells relative to all CK negative cells. (ns = non-significant; ** = p < 0.01; *** = p < 0.001).

Journal: Scientific Reports

Article Title: Evidence for a non-stochastic two-field hypothesis for persistent skin cancer risk

doi: 10.1038/s41598-020-75864-2

Figure Lengend Snippet: Persistent hyperemic foci exhibit evidence of dermal senescence. ( A – E ) Dermal cells in areas of high Hgb content exhibit a heterochromatin pattern of DAPI staining. After excision of skin from mice treated with and without UV at 2 and 20 weeks after stopping UV treatments, the sections were stained with DAPI to better demonstrate nuclear morphology. Nuclei of dermal cells showing a heterochromatin staining pattern are shown by white arrows while nuclei exhibiting a euchromatin pattern of DAPI staining are shown by yellow arrows. The hatched white line outlines the epidermal-dermal junction. The white scale bar represents 50 µm. ( A ) Skin from an area of low Hgb content 2 weeks after stopping UV treatments. ( B ) Skin from an area of high Hgb content 2 weeks after stopping UV treatments. ( C ) Control non-UV treated skin. ( D ) Skin from an area of low Hgb content 20 weeks after stopping UV. ( E ) Skin from an area of high Hgb content 20 weeks after stopping UV. (F – J ) Representative photomigrographs of skin following immunolabeling with anti-p16 INK4a antibodies. ( F ) Control non-UV treated epidermis. ( G , H ) Skin excised 2 weeks after stopping UV treatments from an area of low Hgb content ( G ) or from an area of high Hgb content ( H ). ( I , J ) Skin excised from a low Hgb area ( I ) or a high Hgb area ( J ) at 20 weeks after stopping UV treatments. Black arrows in ( H , J ) show dermal cells with enlarged nuclei labeling positive for p16 INK4a . The black scale bars represent 100 µm. ( K ) HP1γ + dermal cells are increased in hyperemic areas at both 2 and 20 weeks after stopping UV treatments. Immunofluorescent (IF) labeling of formalin-fixed skin sections was performed using anti-HP1γ and anti-pancytokeratin (CK) antibody. The data depicts the % of dermal cells positive for HP1γ nuclear labeling. ( L ) Dermal cells positive for nuclear γH2AX are also increased in hyperemic areas only at 2 and 20 weeks post-UV. IF was performed for both nuclear γH2AX immunolabeling and CK. The data shown is the percentage of γH2AX + dermal cells relative to all CK negative cells. (ns = non-significant; ** = p < 0.01; *** = p < 0.001).

Article Snippet: Immunofluorescent staining of formalin-fixed paraffin-embedded mouse skin following heat-induced antigen retrieval was performed using the following antibodies: HP1γ (Cbx3) immunolabeling was done using rabbit anti-Cbx3 (1:250, Cat#IHC-00204, Bethyl Laboratories). γH2AX immunolabeling was done using monoclonal rabbit anti-γH2AX (1:100, Bethyl Laboratories, Cat#A700-053).

Techniques: Staining, Control, Immunolabeling, Labeling

In vitro validation of differentially expressed proteins. In line with the results of the SILAC method and mass spectrometry, CD81 and CBX-3 show markedly higher expression in TCam-2 than in NTERA-2 (a, b). Conversely, PHF-6 and ENSA show markedly higher expression in NTERA-2 than in TCam-2 cells (c, d).

Journal: Disease Markers

Article Title: Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines

doi: 10.1155/2019/8298524

Figure Lengend Snippet: In vitro validation of differentially expressed proteins. In line with the results of the SILAC method and mass spectrometry, CD81 and CBX-3 show markedly higher expression in TCam-2 than in NTERA-2 (a, b). Conversely, PHF-6 and ENSA show markedly higher expression in NTERA-2 than in TCam-2 cells (c, d).

Article Snippet: For western blotting, the following primary antibody dilutions were used: monoclonal mouse anti-CD81 (Tetraspanin-28) (Santa Cruz, sc-166029; 1 : 250), monoclonal mouse anti-PHF6 (PHD finger protein 6) (Santa-Cruz, sc-365237; 1 : 500), polyclonal rabbit anti-CBX-3 (chromobox protein homolog 3) (HPA 004902, Sigma-Aldrich; 1/250), and polyclonal rabbit anti-ENSA (alpha-endosulfine) (HPA 051292, Sigma-Aldrich: 1/500).

Techniques: In Vitro, Mass Spectrometry, Expressing

Immunohistochemical analysis of selected proteins (more highly expressed in TCam-2 than in NTERA-2) in tumor-free testis, GCNIS, seminoma, and embryonal carcinoma: a tumor-free testis and GCNIS show no differences in CD81 and CBX-3 >expression (a, b, f, g). The expression of CD81 and CBX-3 in embryonal carcinomas (d, i) is significantly lower than that in seminomas (c, h). The differences between seminomas and embryonal carcinomas in staining intensity (IRS) of CD81 (i) and CBX-3 (j) are significant.

Journal: Disease Markers

Article Title: Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines

doi: 10.1155/2019/8298524

Figure Lengend Snippet: Immunohistochemical analysis of selected proteins (more highly expressed in TCam-2 than in NTERA-2) in tumor-free testis, GCNIS, seminoma, and embryonal carcinoma: a tumor-free testis and GCNIS show no differences in CD81 and CBX-3 >expression (a, b, f, g). The expression of CD81 and CBX-3 in embryonal carcinomas (d, i) is significantly lower than that in seminomas (c, h). The differences between seminomas and embryonal carcinomas in staining intensity (IRS) of CD81 (i) and CBX-3 (j) are significant.

Article Snippet: For western blotting, the following primary antibody dilutions were used: monoclonal mouse anti-CD81 (Tetraspanin-28) (Santa Cruz, sc-166029; 1 : 250), monoclonal mouse anti-PHF6 (PHD finger protein 6) (Santa-Cruz, sc-365237; 1 : 500), polyclonal rabbit anti-CBX-3 (chromobox protein homolog 3) (HPA 004902, Sigma-Aldrich; 1/250), and polyclonal rabbit anti-ENSA (alpha-endosulfine) (HPA 051292, Sigma-Aldrich: 1/500).

Techniques: Immunohistochemical staining, Expressing, Staining

Transfection with siRNA markedly reduces protein expression of CD81, CBX-3, PHF-6, and ENSA: NCCIT, NTERA-2, and TCam-2 were transfected with a siRNAs against CD81, CBX-3, PHF-6, and ENSA. The protein expression was markedly reduced after transfection with siRNA (a–d).

Journal: Disease Markers

Article Title: Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines

doi: 10.1155/2019/8298524

Figure Lengend Snippet: Transfection with siRNA markedly reduces protein expression of CD81, CBX-3, PHF-6, and ENSA: NCCIT, NTERA-2, and TCam-2 were transfected with a siRNAs against CD81, CBX-3, PHF-6, and ENSA. The protein expression was markedly reduced after transfection with siRNA (a–d).

Article Snippet: For western blotting, the following primary antibody dilutions were used: monoclonal mouse anti-CD81 (Tetraspanin-28) (Santa Cruz, sc-166029; 1 : 250), monoclonal mouse anti-PHF6 (PHD finger protein 6) (Santa-Cruz, sc-365237; 1 : 500), polyclonal rabbit anti-CBX-3 (chromobox protein homolog 3) (HPA 004902, Sigma-Aldrich; 1/250), and polyclonal rabbit anti-ENSA (alpha-endosulfine) (HPA 051292, Sigma-Aldrich: 1/500).

Techniques: Transfection, Expressing

Transfection with siRNA decreases cell proliferation of GCT cell lines: in all investigated tumor cell lines, NCCIT (a), NTERA-2 (b), and TCam-2 (c), proliferation was significantly reduced after transfection with siRNA against CD81, CBX-3, PHF-6, and ENSA.

Journal: Disease Markers

Article Title: Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines

doi: 10.1155/2019/8298524

Figure Lengend Snippet: Transfection with siRNA decreases cell proliferation of GCT cell lines: in all investigated tumor cell lines, NCCIT (a), NTERA-2 (b), and TCam-2 (c), proliferation was significantly reduced after transfection with siRNA against CD81, CBX-3, PHF-6, and ENSA.

Article Snippet: For western blotting, the following primary antibody dilutions were used: monoclonal mouse anti-CD81 (Tetraspanin-28) (Santa Cruz, sc-166029; 1 : 250), monoclonal mouse anti-PHF6 (PHD finger protein 6) (Santa-Cruz, sc-365237; 1 : 500), polyclonal rabbit anti-CBX-3 (chromobox protein homolog 3) (HPA 004902, Sigma-Aldrich; 1/250), and polyclonal rabbit anti-ENSA (alpha-endosulfine) (HPA 051292, Sigma-Aldrich: 1/500).

Techniques: Transfection